NR1H2 Knockout cell line (Vips)

NR1H2 Knockout cell line (Vips) Order

Catalog#

YKO-H879

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

Detailed Product Information

Product category

KO Cell Lines

cell name

NR1H2 Knockout cell line（IPSC-DYR0100）

Cell Morphology

Spherical clone

Passage ratio

1:6-1:8

Species

Human

Gene Name

NR1H2

Gene ID

7376

Gene Information

NCBI

Construction method

Electroporation/Lentivirus

Mycoplasma test

negative

Culture method

EZ-Stem™ Cell Culture Medium

Target region

Knockout region: Exon 3,Exon 4 (based on transcript NR1H2-201)
Validation: PCR+Sanger Sequencing

STR Profile

Loci	STR Info (Sample Cell) Sample Cell Line:IPSC-DYR0100			STR Info (Cell bank) Cell Line:ATCC- DYR0100 hiPSC
Loci	Allele1	Allele2	Allele3	Allele1	Allele2	Allele3
D5S818	11	11		11	11
D13S317	11	13		11	13
D7S820	9	10		9	10
D16S539	12	12		12	12
VWA	14	19		14	19
TH01	9.3	9.3		9.3	9.3
AMEL	X	Y		X	Y
TPOX	8	11		8	11
CSF1PO	7	11		7	11
D12S391	18	19.3
FGA	21	22
D2S1338	17	19
D21S11	30	32.2
D18S51	10	13
D8S1179	14	15
D3S1358	15	17
D6S1043	12	13
PENTAE	7	7
D19S433	14	15
PENTAD	12	13
D1S1656	12	17.3

* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the NR1H2 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the IPSC-DYR0100 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, NR1H2 gene knockout NR1H2 Knockout cell line（IPSC-DYR0100） will be expanded and cryopreserved.

Knockout Cell Line Work Flow and Validatio

NR1H2 Knockout cell line (Vips)

Catalog#

YKO-H879

Size

1*10^6

Storage conditions

Shipped on dry ice; Store in liquid nitrogen

Order

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NR1H2 Knockout cell line (Vips)

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